t2a egfp Search Results


panx flx flx mice  (Vector Biolabs)
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burs mcd8 egfp t2a gal4  (Addgene inc)
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cell line t 2a egfp pgk puro  (Addgene inc)
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cag cas9 t2a egfp ires puro plasmid  (Addgene inc)
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pcxle dcas9vp192 t2a egfp shp53  (Addgene inc)
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sgrna  (Addgene inc)
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Addgene inc sgrna
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aav9 ctnt egfp t2a icre wpre  (Vector Biolabs)
90
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gcn4 fragment  (Addgene inc)
92
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cag dcas9vp192 t2a gfp ires puro backbone  (Addgene inc)
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Cag Dcas9vp192 T2a Gfp Ires Puro Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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minisog2  (Addgene inc)
92
Addgene inc minisog2
( A ) Swimming activity of <t>Tg(elavl3:sypb-miniSOG2-P2A-mScarlet)</t> zebrafish at 110 hpf was tracked pre- and post-blue light exposure for 5 min. Green lines show tracks at the end of the time-lapse. ( B, C ) Quantification of swimming activity of Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) zebrafish at 110 hpf; mean ± standard error of the mean (SEM), n = 21–23 animals, p values from two-way analysis of variance (ANOVA) with Holm–Sidak’s multiple comparisons test. ( D ) Schematic of optogenetic neural inhibition experiment.
Minisog2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flippase expressing cassette  (Vector Biolabs)
95
Vector Biolabs flippase expressing cassette
( A ) Swimming activity of <t>Tg(elavl3:sypb-miniSOG2-P2A-mScarlet)</t> zebrafish at 110 hpf was tracked pre- and post-blue light exposure for 5 min. Green lines show tracks at the end of the time-lapse. ( B, C ) Quantification of swimming activity of Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) zebrafish at 110 hpf; mean ± standard error of the mean (SEM), n = 21–23 animals, p values from two-way analysis of variance (ANOVA) with Holm–Sidak’s multiple comparisons test. ( D ) Schematic of optogenetic neural inhibition experiment.
Flippase Expressing Cassette, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cre recombinase  (Vector Biolabs)
95
Vector Biolabs cre recombinase
( A ) Swimming activity of <t>Tg(elavl3:sypb-miniSOG2-P2A-mScarlet)</t> zebrafish at 110 hpf was tracked pre- and post-blue light exposure for 5 min. Green lines show tracks at the end of the time-lapse. ( B, C ) Quantification of swimming activity of Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) zebrafish at 110 hpf; mean ± standard error of the mean (SEM), n = 21–23 animals, p values from two-way analysis of variance (ANOVA) with Holm–Sidak’s multiple comparisons test. ( D ) Schematic of optogenetic neural inhibition experiment.
Cre Recombinase, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Swimming activity of Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) zebrafish at 110 hpf was tracked pre- and post-blue light exposure for 5 min. Green lines show tracks at the end of the time-lapse. ( B, C ) Quantification of swimming activity of Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) zebrafish at 110 hpf; mean ± standard error of the mean (SEM), n = 21–23 animals, p values from two-way analysis of variance (ANOVA) with Holm–Sidak’s multiple comparisons test. ( D ) Schematic of optogenetic neural inhibition experiment.

Journal: eLife

Article Title: Innervation modulates the functional connectivity between pancreatic endocrine cells

doi: 10.7554/eLife.64526

Figure Lengend Snippet: ( A ) Swimming activity of Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) zebrafish at 110 hpf was tracked pre- and post-blue light exposure for 5 min. Green lines show tracks at the end of the time-lapse. ( B, C ) Quantification of swimming activity of Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) zebrafish at 110 hpf; mean ± standard error of the mean (SEM), n = 21–23 animals, p values from two-way analysis of variance (ANOVA) with Holm–Sidak’s multiple comparisons test. ( D ) Schematic of optogenetic neural inhibition experiment.

Article Snippet: The Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) line was generated by Tol2 transgenesis of a 8.7 kb elavl3 promoter (Addgene: 59531, AgeI restriction enzyme digested) driving expression of sypb (Addgene: 74316), miniSOG2 (Addgene: 87410), P2A-mScarlet (gift from A. Beisaw) cloned with Cold Fusion (System Biosciences).

Techniques: Activity Assay, Inhibition

( A ) Whole larva-free glucose-level measurements of Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) zebrafish at 110 hpf; mean ± standard error of the mean (SEM), n = 22–31 batches of 5 larvae per replicate, p values from two-way analysis of variance (ANOVA) with Holm–Sidak’s multiple comparisons test; see . ( B ) Normalized calcium traces of pancreatic islet cells (including delta, beta, alpha, and unidentified cells). Individual islet cells were assigned to a cell type and given a cell id; LG, basal condition; HG, glucose treated condition. ( C ) Correlation matrices of islet cell activity; LG, basal condition; HG, glucose treated condition; HG λ, post-blue light treatment. ( D ) Average beta cell correlation coefficients in individual larvae; n = 10 animals, p values from two-way ANOVA with Holm–Sidak’s multiple comparisons test. ( E ) Average homotypic (beta–beta) and heterotypic (alpha–beta) cell correlation coefficients with cell distance distribution from 1 (close) to 10 (far), mean and linear regression (solid lines) with 95% confidence intervals; n = 10 animals, p values of slope and intercept from simple linear regression. Fraction time analysis of heterotypic delta–beta, alpha–delta, and alpha–beta cell pairs for times when both are active ( F ), both are silent ( G ), and one is active and one is silent ( H ); mean ± SEM, n = 141–220 cell pairs, p values from two-way ANOVA with Holm–Sidak’s multiple comparisons test; magenta circle, active state; cyan circle, silent state. Figure 5—source data 1. . Whole larva-free glucose-level measurements of Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) zebrafish.

Journal: eLife

Article Title: Innervation modulates the functional connectivity between pancreatic endocrine cells

doi: 10.7554/eLife.64526

Figure Lengend Snippet: ( A ) Whole larva-free glucose-level measurements of Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) zebrafish at 110 hpf; mean ± standard error of the mean (SEM), n = 22–31 batches of 5 larvae per replicate, p values from two-way analysis of variance (ANOVA) with Holm–Sidak’s multiple comparisons test; see . ( B ) Normalized calcium traces of pancreatic islet cells (including delta, beta, alpha, and unidentified cells). Individual islet cells were assigned to a cell type and given a cell id; LG, basal condition; HG, glucose treated condition. ( C ) Correlation matrices of islet cell activity; LG, basal condition; HG, glucose treated condition; HG λ, post-blue light treatment. ( D ) Average beta cell correlation coefficients in individual larvae; n = 10 animals, p values from two-way ANOVA with Holm–Sidak’s multiple comparisons test. ( E ) Average homotypic (beta–beta) and heterotypic (alpha–beta) cell correlation coefficients with cell distance distribution from 1 (close) to 10 (far), mean and linear regression (solid lines) with 95% confidence intervals; n = 10 animals, p values of slope and intercept from simple linear regression. Fraction time analysis of heterotypic delta–beta, alpha–delta, and alpha–beta cell pairs for times when both are active ( F ), both are silent ( G ), and one is active and one is silent ( H ); mean ± SEM, n = 141–220 cell pairs, p values from two-way ANOVA with Holm–Sidak’s multiple comparisons test; magenta circle, active state; cyan circle, silent state. Figure 5—source data 1. . Whole larva-free glucose-level measurements of Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) zebrafish.

Article Snippet: The Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) line was generated by Tol2 transgenesis of a 8.7 kb elavl3 promoter (Addgene: 59531, AgeI restriction enzyme digested) driving expression of sypb (Addgene: 74316), miniSOG2 (Addgene: 87410), P2A-mScarlet (gift from A. Beisaw) cloned with Cold Fusion (System Biosciences).

Techniques: Activity Assay

List of zebrafish transgenic lines.

Journal: eLife

Article Title: Innervation modulates the functional connectivity between pancreatic endocrine cells

doi: 10.7554/eLife.64526

Figure Lengend Snippet: List of zebrafish transgenic lines.

Article Snippet: The Tg(elavl3:sypb-miniSOG2-P2A-mScarlet) line was generated by Tol2 transgenesis of a 8.7 kb elavl3 promoter (Addgene: 59531, AgeI restriction enzyme digested) driving expression of sypb (Addgene: 74316), miniSOG2 (Addgene: 87410), P2A-mScarlet (gift from A. Beisaw) cloned with Cold Fusion (System Biosciences).

Techniques: Transgenic Assay, Marker